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. 2022 May 5;13:885053. doi: 10.3389/fphar.2022.885053

FIGURE 6.

FIGURE 6

The Syk inhibitor R406 enhances the inhibitory effect of dexamethasone on transrepression and transactivation. (A) BEAS-2B cells were pretreated with R406 (1 μM) for 30 min, incubated with or without Dex (1 μM) for 2 h, and stimulated with TNF-α (10 ng/ml) for 10 min (B) BEAS-2B cells were pretreated with R406 (1 μM) for 30 min and incubated with TNF-α (10 ng/ml) or with Dex (1 μM) and TNF-α for 12 h. (C) The expression of HDAC2 in asthmatic mouse lung tissues was determined by Western blotting. n = 5/group. (D,E) BEAS-2B cells were pretreated with R406 (1 μM) for 30 min, incubated with Dex (1 μM) for 2 h and stimulated with or without TNF-α (10 ng/ml) for 24 h, and the mRNA levels of GILZ and CLCCI1 were measured by PCR. n = 3/group, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, compared with the control group; ## p < 0.01, #### p < 0.0001, compared with the TNF-α or asthma model group; ▫p < 0.05 compared with the Dex group; △ p < 0.05, △△ p < 0.01, compared with the TNF-α +Dex group or asthma + Dex treatment group.