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. 2022 May 17;219(6):e20212019. doi: 10.1084/jem.20212019

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© 2022 Hasebe et al.

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Figure S1. — Phenotypes of cytokine-induced arthritis with activation of sensory neurons in L5 DRG. (A) Histological analysis of the left ankle joint of F759 mice on day 7 with saline or cytokine injections (IL-17A and IL-6 [0.1 μg each]) on days 0, 1, and 2. Experiments were performed at least three times independently; representative data are shown. (B) Immunostaining of MHC II, CD11b, and CD4 in the ankle joints of F759 mice with saline or cytokine injection (IL-17 and IL-6 [0.1 μg each]) bilaterally on days 0, 1, and 2. Experiments were performed three times independently. Bars, 1 mm and 50 μm. MHC II+, CD11b+, and CD4+ areas were quantified from the left or right ankle joints of mice for different clinical scores (n = 3 each). The data are shown as means ± SEM. P values were calculated using Dunnett’s test. (C) FG was injected into the right ankle joint of F759 mice, followed by the detection of FG and staining of Nav1.8 and TRPV1 in the ipsilateral (right, R) and contralateral (left, L) L5 DRG on day 7. The number of FG+ cells, Nav1.8+TRPV1−, Nav1.8+TRPV1+, and Nav1.8−TRPV1+ cells was counted (n = 3 for the upper graph, n = 6 for the lower graph). The upper graph shows the percentage of FG+ cells in the total number of Nav1.8+TRPV1−, Nav1.8+TRPV1+, and Nav1.8−TRPV1+ cells. The lower graph shows the percentage of Nav1.8+TRPV1−, Nav1.8+TRPV1+, and Nav1.8−TRPV1+ cells in the total number of DRG neurons. Data represent the mean ± SD. Magenta, FG. Green, Nav1.8. Red, TRPV1. Blue, Nuclei. Bar, 100 µm. The diagram illustrates the experimental settings. L, left ankle; R, right ankle. Arrow indicates FG injection, and the red circles indicate the L5 DRGs examined.