Fig. 4.

Mutation signature analysis. (A) The distribution of each mutation signature in 97 glioma samples. The upper panel showed the mutation counts attributed to different signatures. The middle panel showed the percent. The lower panel showed the somatic mutation of the MMR encoding genes and clinical information. (B) Stacked bar plot showing the proportion of each mutation signature that was shared, primary-private or recurrent-private. The difference between the spectra for three groups across all cases was assessed using a χ2 test. C. 96-trinucleotide-motif plot of all single base substitutions (SBSs) in hypermutated cases. The pie plot showed the proportion of various mutational signatures. (D-E) Distribution of mutational signatures associated with driver gene mutations. The bar plots showed the cumulative probabilities of each driver gene mutation being due to each mutational process. The pie charts showed the percentage of mutations signatures across all driver gene mutations. (F-G) Comparison of subclonal(later)-to-clonal(earlier) mutation fold change caused by each mutation signature between GBMs and IDH mutant gliomas. Each sample was represented as a dot. RT: Radiotherapy; TMZ: Temozolomide; Pri: Primary gliomas; Rec: Recurrent gliomas.