Fig. 5.

Characteristics of neoantigens among primary and recurrent glioma. (A) Box plot and and paired lines showing comparison of the number of neoantigens between primary and recurrent samples. Wilcoxon signed-rank test P values were indicated. (B) Scatter plot showing the relationship between the number of neoantigens of primary and recurrent gliomas. Spearman's rank correlation coefficient and P values were indicated. (C) Box plot and and paired lines showing comparison of mean DAI of neoantigen between primary and recurrent gliomas across two subtypes. (D) Percent stacked bar chart showing the proportion of high the TCR recognition. (E) The proportion of neoantigens arising from clonal or subclonal mutations was shown in primary and recurrent gliomas. (F) Percent stacked bar chart showing the different proportions of copy number loss related neoantigen among primary and recurrent gliomas. (G) Number and distribution of neoantigens produced by different mutational processes. (H) Comparison of TCR recognition probability and clonality of neoantigens produced in different mutational processes. Statistical tests used can be found in materials and methods. HM: Hypermutation RT: Radiotherapy; TMZ: Temozolomide; Pri: Primary gliomas; Rec: Recurrent gliomas.