Figure 3.

β-cell-specific LRH-1 ablation in adult mice negates the antidiabetic properties of BL001

(A and B) Diabetes incidence of IndβLRH-1 mice either (A) not treated or (B) treated with TAM and then subjected to an STZ and/or BL001 regimen for 5 weeks. BL001 (10 mg/kg body weight) treatment started 4 weeks after finishing TAM treatment. Diabetes was induced at week one post-BL001 treatment with a single high dose of STZ (175 mg/kg body weight).

(C) Representative immunofluorescence images of pancreas sections from the various experimental groups co-stained for insulin (INS, red) and glucagon (GLUC, green). Nuclei were stained with DAPI (blue). Scale bar: 50 μM.

(D–F) Quantification of (D) β-cells, (E) α-cells and (F) ratio thereof in the different groups. n = individual islets counted from three to six independent mice. Statistics: Data are represented as the mean ± SEM ∗p < 0.05 and ∗∗∗∗p < 0.0001, unpaired Student’s t test between (−) and (+) TAM provided in the various treatments.

See also Figure S2.