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. 2022 May 2;25(5):104345. doi: 10.1016/j.isci.2022.104345

Figure 5.

Figure 5

PTGS2 silenced islets are refractory to the protective effect of BL001 under cytokine attack

(A and B) Representative immunofluorescence images of embedded islets transfected with either a control siRNA (siSC) or siPTGS2 and treated or not with cytokines (CTK) or/and with BL001. Islet slices were stained for either (A) Cytochrome c (CytoC, green) or (B) cleaved PARP (Cl.PARP, green) along with insulin (INS, red). Nuclei were stained with DAPI (blue). Scale bar: 25 μM.

(C and D) Protein levels of cleaved PARP (Cl.PARP) (C) and (D) quantification thereof were assessed in whole islet cell extracts that were transfected with either a control siRNA (siSC) or siPTGS2 and treated or not with CTK or/and with BL001. Relative protein levels were normalized to ACT. Each line represents an independent experiment. Results are expressed as means ± SEM ∗∗p < 0.01 unpaired two-tailed t-test siSC/CTK versus siSC/CTK/BL001.

(E) Transcript levels of Bax were also determined in the same experimental groups. Expression levels were normalized to the housekeeping gene Cyclophilin. Results are expressed as means ± SEM ∗∗∗∗p < 0.0001, unpaired two-tailed t-test siCT/CTK versus siSC/CTK/BL001.