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Preparation of MethoCult H4230/cell mixture
| Reagent | Volume for duplicates | Volume for triplicates |
|---|---|---|
| MethoCult (H4230) | 2.4 mL | 3.2 mL |
| Cells in IMDM∗ | 300 μL | 400 μL |
| Cytokines in IMDM∗∗ | 600 μL | 800 μL |
| Antibiotic-Antimycotic | 30 μL | 40 μL |
| Total | 3.33 mL | 4.44 mL |
∗ Cell concentration should be 10 times higher than a final concentration. 1,000 cells/dish in a final concentration corresponds to 10,000 cells/mL in IMDM.
∗∗ Cytokine concentration should be 5 times higher than final concentration.
