REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, peptides, and recombinant proteins
D-MEM F12	Sigma	Cat# D6421
KnockOut SR	Gibco	Cat# 10828-028
NEAA	Gibco	Cat# 11140-035
GlutaMAX	Gibco	Cat# 35050061
2-Mercapto Ethanol	Gibco	Cat# 31350-010
Penicillin/Streptomycin	Sigma	Cat# P0781
PBS	Gibco	Cat# 14190-094
EDTA 0.5 M	Sigma	Cat# E7889
bFGF (FGF2)	PeproTech	Cat# 100-18B
BMP4	R&D	Cat# 314-BP
VEGF	R&D	Cat# 293-VE
SCF	PeproTech	Cat# 300-07
TPO	PeproTech	Cat# 300-18
FLT3L	BioLegend	Cat# 550 606
IL-3	PeproTech	Cat# 200-03
IL-6	PeproTech	Cat# 200-06
Rock-inhibitor Y-27632	Tocris	Cat# 1254
Geltrex	Thermo Fisher Scientific	Cat# A1413202
STEMdiff APEL2 Medium	STEMCELL Technologies	Cat# 05270
MethoCult H4435	STEMCELL Technologies	Cat# 04435
MethoCult H4230	STEMCELL Technologies	Cat# 04230
IMDM with 2% FBS	STEMCELL Technologies	Cat# 07700
Antibiotic-Antimycotic	Thermo Fisher Scientific	Cat# 15240-096
May-Grünwald-Solution	Merck	Cat# 101424
Giemsa-Solution	Merck	Cat# 109204
0,05% Trypsin-EDTA	Gibco	Cat# 25300-054
FBS	Sigma	Cat# 7524
G418, Geneticin	Thermo Fisher Scientific	Cat# 10131035
DMEM (1×)	Gibco	Cat# 41966-052
Bovine Collagen Solution Typ I, 3 mg/mL	Sigma	Cat# 804592
Mitomycin C	Sigma	Cat# M0503
DMSO	Sigma	Cat# D8418
MyeloCult H5100	STEMCELL Technologies	Cat# 05150
Hydrocortisone 100 mg	STEMCELL Technologies	Cat# 74142
CTS PSC Cryomedium	STEMCELL Technologies	Cat# A4238801
Stem Span SFEM II	STEMCELL Technologies	Cat# 09655
7AAD	BD Biosciences	Cat# 559925
Antibodies
CD34 - PE-Cy7	BD Biosciences	Cat# 348811
CD45 - BV510	BioLegend	Cat# 304036
Experimental models: Cell lines
SNL-feeder cells	Public Health England	Cat# 07032801
Sl/Sl (FLT3L) Cell Line	Connie Eaves, Terry Fox Laboratory, BC, Canada or STEMCELL Technologies	N/A contact techsupport@stemcell.com
Sl/Sl (IL-3, SCF) Cell Line	STEMCELL Technologies	Cat# 00302
Other
Blunt-End Needles, 16 Gauge	STEMCELL Technologies	Cat# 28110
Microscope slides	R. Langenbrink	Cat# 300030
Mr. Frosty Freezing Container	Thermo Fisher Scientific	Cat# 5100-0001