Figure 1.

Analysis of GC B cells isolated from naive and immunized mice

(A) Pre and Post Sort flow cytometry analysis of purified GC B cells from naive mice (left) and immunized mice (right). B cells are identified as B220⁺, and GC B cells are identified as B220⁺FAS⁺GL7⁺.

(B) Bar graph of compiled flow cytometry data depicting means with SEM error bars; 2-tailed T test; n = 8 biological replicates of naive and immunized mice.

(C) Bar graph showing the proportions of B220⁺ and CD43⁺ cells prior to sorting in naive and immunized mice, depicting means with SEM error bars; 2-tailed T test; n= 12 biological replicates for immunized mice; n = 13 biological replicates for naive mice.