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. 2022 May 14;3(2):101388. doi: 10.1016/j.xpro.2022.101388

10× Annexin V Stock Solution

Reagent Final concentration Amount
HEPES 1 M pH 7.3 0.1 M 5 mL
NaCl 1.4 M 4.09 g
CaCl2.H2O 25 mM 183.75 mg
ddH20 n/a 45 mL
Total 50 mL

Storage at –4°C, discard if solution begins to precipitate.