REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Biotin Rat Anti-Mouse CD43 (S7) (1:50 dilution) | BD Pharmingen | Cat# 553269 RRID: AB_2255226 |
Anti-Mouse IgD (11-26c) Biotin (1:25 and 1:50 dilution) | Thermo Fisher Scientific | Cat# 13-5993-82, RRID: AB_466860 |
Anti-Mouse CD38 (90) Biotin (1:50 dilution) | Thermo Fisher Scientific | Cat# 13-0381-82 RRID: AB_466428 |
Anti-Mouse CD11c (N418) Biotin (1:50 dilution) | Thermo Fisher Scientific | Cat# 13-0114-82 RRID: AB_466363 |
Anti-Mouse TER119 (TER-119) Biotin (1:60 dilution) | Thermo Fisher Scientific | Cat# 13-5921-82 RRID: AB_466797 |
Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5) Biotin (1:60 dilution) | Thermo Fisher Scientific | Cat#: 13-5931-82 RRID: AB_466800 |
Anti-Hu/Mo GL7 (GL-7) AF488 (1:100 dilution) | Thermo Fisher Scientific | Cat# 53-5902-82 RRID: AB_2016717 |
PE Hamster Anti-Mouse CD95 (Jo2) (1:100 dilution) | BD Pharmingen | Cat#: 554258 RRID: AB_395330 |
FITC Rat Anti-Mouse T- and B- Cell Activation Antigen (GL7) (1:100 dilution) | BD Pharmingen | Cat# 562080 RRID: AB_394981 |
PE-Cy5 Rat Anti-Mouse CD45R/B220 (RA3-6B2) (1:100 dilution) | BD Pharmingen | Cat# 553091 RRID: AB_394621 |
Biological samples | ||
Citrated Sheep Red Blood Cells | Colorado Serum Company | Cat# 31102 |
Chemicals, peptides, and recombinant proteins | ||
Biotin Annexin V (1:50 dilution) | BD Pharmingen | Cat# 556417 RRID: AB_2869070 |
FITC Annexin V (1:100 dilution) | BD Pharmingen | Cat# BDB556420 |
PE Annexin V (1:100 dilution) | BioLegend | Cat# 640947 |
APC Annexin V (1:100 dilution) | BD Pharmingen | Cat# 561012 RRID: AB_2868885 |
Propidium Iodide Staining Solution (1:100 dilution) | BD Pharmingen | Cat# 556463 RRID: AB_2869075 |
7-AAD Viability Staining Solution (1:100 dilution) | eBioscience | Cat# 00-6993-50 |
Annexin V Binding Buffer, 10× concentrate | BD Pharmingen | Cat# 556454 RRID: AB_2869074 |
Dynabeads Mouse CD43 (Untouched B cells) | Invitrogen | Cat# 11422D |
Dynabeads Biotin Binder | Invitrogen | Cat# 11047 |
DNase I | Roche | Cat# 10104159001 |
Bovine Serum Albumin (BSA) | Fisher Scientific | Cat# BP1605-100 |
Fetal Bovine Serum (Heat Inactivated) | Gibco | Ref# 10082-147 |
PBS pH 7.4 (1×) (-CaCl2) (-MgCl2) | Gibco | Ref# 10010-031 |
eBioscience 1× RBC Lysis Buffer | Invitrogen | Ref# 00-4333-57 |
HEPES Buffer, 1 M Solution | Fisher | Cat# BP299-100 |
Ammonium Chloride | Fisher | Cat# A661-500 |
Potassium Bicarbonate | Fisher | Cat# P235-500 |
Sodium Chloride | Fisher | Cat# BP358-10 |
Magnesium Chloride | Fisher | Cat# M33-500 |
Calcium Chloride Dihydrate | Sigma | Cat# C5080 |
Critical commercial assays | ||
Debris Removal Solution | Miltenyi Biotec | Cat# 130-109-398 |
Experimental models: Organisms/strains | ||
Mouse: 3 to 6-month-old male and female wild type C57BL/6 | In house breeding, originally purchased from JAX lab | N/A |
Mouse: 4-6-month-old female NZBWF1 | JAX lab | Strain#: 100008 RRID: IMSR_JAX:100008 |
Software and algorithms | ||
NovoExpress | Agilent | N/A |
Excel | Microsoft | N/A |
PowerPoint | Microsoft | N/A |
Illustration | BioRender | N/A |
Other | ||
Magnetic Particle Concentrator (Dynal MPC-L) | Dynal | Product# 120.21 |
Novocyte | Agilent | N/A |
Eppendorf Centrifuge 5417R | Eppendorf | N/A |
Eppendorf Centrifuge 5430R | Eppendorf | N/A |
Sorvall ST 16R Centrifuge | Thermo Scientific | N/A |
Frosted Microscope Slides | Fisher Scientific | Cat# 12-550-343 |
Cell Strainer 70 μm Nylon | Corning Incorporated | Ref# 352350 |
Labquake Rotator/Shaker | Barnstead Thermolyne | Model 40011 |