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. 2022 May 14;3(2):101388. doi: 10.1016/j.xpro.2022.101388
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Biotin Rat Anti-Mouse CD43 (S7) (1:50 dilution) BD Pharmingen Cat# 553269 RRID: AB_2255226
Anti-Mouse IgD (11-26c) Biotin (1:25 and 1:50 dilution) Thermo Fisher Scientific Cat# 13-5993-82, RRID: AB_466860
Anti-Mouse CD38 (90) Biotin (1:50 dilution) Thermo Fisher Scientific Cat# 13-0381-82
RRID: AB_466428
Anti-Mouse CD11c (N418) Biotin (1:50 dilution) Thermo Fisher Scientific Cat# 13-0114-82
RRID: AB_466363
Anti-Mouse TER119 (TER-119) Biotin (1:60 dilution) Thermo Fisher Scientific Cat# 13-5921-82
RRID: AB_466797
Anti-Mouse Ly-6G/Ly-6C (Gr-1) (RB6-8C5) Biotin (1:60 dilution) Thermo Fisher Scientific Cat#: 13-5931-82
RRID: AB_466800
Anti-Hu/Mo GL7 (GL-7) AF488 (1:100 dilution) Thermo Fisher Scientific Cat# 53-5902-82
RRID: AB_2016717
PE Hamster Anti-Mouse CD95 (Jo2) (1:100 dilution) BD Pharmingen Cat#: 554258
RRID: AB_395330
FITC Rat Anti-Mouse T- and B- Cell Activation Antigen (GL7) (1:100 dilution) BD Pharmingen Cat# 562080
RRID: AB_394981
PE-Cy5 Rat Anti-Mouse CD45R/B220 (RA3-6B2) (1:100 dilution) BD Pharmingen Cat# 553091
RRID: AB_394621

Biological samples

Citrated Sheep Red Blood Cells Colorado Serum Company Cat# 31102

Chemicals, peptides, and recombinant proteins

Biotin Annexin V (1:50 dilution) BD Pharmingen Cat# 556417
RRID: AB_2869070
FITC Annexin V (1:100 dilution) BD Pharmingen Cat# BDB556420
PE Annexin V (1:100 dilution) BioLegend Cat# 640947
APC Annexin V (1:100 dilution) BD Pharmingen Cat# 561012
RRID: AB_2868885
Propidium Iodide Staining Solution (1:100 dilution) BD Pharmingen Cat# 556463
RRID: AB_2869075
7-AAD Viability Staining Solution (1:100 dilution) eBioscience Cat# 00-6993-50
Annexin V Binding Buffer, 10× concentrate BD Pharmingen Cat# 556454
RRID: AB_2869074
Dynabeads Mouse CD43 (Untouched B cells) Invitrogen Cat# 11422D
Dynabeads Biotin Binder Invitrogen Cat# 11047
DNase I Roche Cat# 10104159001
Bovine Serum Albumin (BSA) Fisher Scientific Cat# BP1605-100
Fetal Bovine Serum (Heat Inactivated) Gibco Ref# 10082-147
PBS pH 7.4 (1×) (-CaCl2) (-MgCl2) Gibco Ref# 10010-031
eBioscience 1× RBC Lysis Buffer Invitrogen Ref# 00-4333-57
HEPES Buffer, 1 M Solution Fisher Cat# BP299-100
Ammonium Chloride Fisher Cat# A661-500
Potassium Bicarbonate Fisher Cat# P235-500
Sodium Chloride Fisher Cat# BP358-10
Magnesium Chloride Fisher Cat# M33-500
Calcium Chloride Dihydrate Sigma Cat# C5080

Critical commercial assays

Debris Removal Solution Miltenyi Biotec Cat# 130-109-398

Experimental models: Organisms/strains

Mouse: 3 to 6-month-old male and female wild type C57BL/6 In house breeding, originally purchased from JAX lab N/A
Mouse: 4-6-month-old female NZBWF1 JAX lab Strain#: 100008
RRID: IMSR_JAX:100008

Software and algorithms

NovoExpress Agilent N/A
Excel Microsoft N/A
PowerPoint Microsoft N/A
Illustration BioRender N/A

Other

Magnetic Particle Concentrator (Dynal MPC-L) Dynal Product# 120.21
Novocyte Agilent N/A
Eppendorf Centrifuge 5417R Eppendorf N/A
Eppendorf Centrifuge 5430R Eppendorf N/A
Sorvall ST 16R Centrifuge Thermo Scientific N/A
Frosted Microscope Slides Fisher Scientific Cat# 12-550-343
Cell Strainer 70 μm Nylon Corning Incorporated Ref# 352350
Labquake Rotator/Shaker Barnstead Thermolyne Model 40011