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Example of air-liquid interface (ALI) seeding calculations
| # Cells counted at confluency in T75 | # Cells required for seeding per 24-well insert | # Inserts to be seeded | # Total cells required | # Total cells remaining to freeze | # Cells required for T75 seeding |
|---|---|---|---|---|---|
| 5.5 × 106 | 3 × 105 | 4 | 1.2 × 106 | 4.3 × 106 | 2.1 × 106 |
