Small Airway Epithelial Growth Medium (SAGM) Medium

Component	Final concentration	Amount
SAGM Medium	n/a	500 mL
SAGM Supplement Mix	n/a	22.5 mL
Y-27632 2HCl	5 μM	250 μL
A 83-01	1 μM	50 μL
CHIR 99021	0.4 μM	20 μL
RAPAMYCIN 99%	5 nM	125 μL
Normocin	n/a	1 mL
Total	n/a	523.95 mL

See Figure 2 in Lu et al. (2021) for representative images and proliferation quantification comparing BSC cultures with and without rapamycin.