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PneumaCult Medium with Inhibitors (first 7 days of differentiation)
| Component | Final concentration | Amount |
|---|---|---|
| PneumaCult-ALI Basal Medium | n/a | 500 mL |
| PneumaCult-ALI 10× Supplement | n/a | 50 mL |
| PneumaCult-ALI Maintenance Supplement | n/a | 5 × 1 mL |
| Y-27632 | 5 μM | 250 μL |
| A 83-01 | 0.5 μM | 25 μL |
| Normocin | n/a | 1 mL |
| Total | n/a | 556.3 mL |
