| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-FOXA2 antibody (rabbit) (1:100) | Abcam | Cat# ab23630 |
| NKX2.1 antibody (rabbit) (1:100) | Santa Cruz Biotechnology | Cat# sc-13040 |
| CC10 antibody (E-11) (mouse) (1:100) | Sigma-Aldrich | Cat# HPA031828 |
| Muc5ac Monoclonal antibody (45M1) (mouse) (1:100) | Thermo Scientific | Cat# MA5-12178 |
| p63-α (D2K8X) XP® (rabbit) antibody (1:100) | Cell Signaling Technology | Cat# 13109S |
| p63 (D-9) (mouse) antibody (1:100) | Santa Cruz Biotechnology | Cat# sc-25268 |
| KRT8 antibody (mouse) (1:100) | BioLegend | Cat# 904804 |
| KRT5 antibody (rabbit) (1:100) | Abcam | Cat# ab53121 |
| Biotin anti-human CD326 antibody (EpCAM) (mouse) (1:100) | BioLegend | Cat# 369812 |
| Anti-RFX3 antibody (rabbit) (1:100) | Sigma-Aldrich | Cat# HPA035689 |
| Goat anti Rabbit IgG (H+L) Secondary antibody, Alexa Fluor 594, Invitrogen (1:200) | Thermo Scientific | Cat# A11037 |
| Goat anti Mouse IgM Heavy Chain Secondary antibody, Alexa Fluor 488, Invitrogen (1:200) | Thermo Scientific | Cat# A-21042 |
| Monoclonal Anti-Tubulin, antibody (mouse) (1:100) | Sigma-Aldrich | Cat# T6793 |
| DAPI Solution (1 mg/mL) | Thermo Scientific | Cat# 62248 |
| Anti-Biotin Microbeads | Miltenyi Biotec | Cat# 130-090-485 |
| Sucrose, for molecular biology, ≥99.5% (GC) | Sigma-Aldrich | Cat# S0389 |
| Chemicals, peptides, and recombinant proteins | ||
| Ethanol | Fisher Scientific | Cat# BP2818-4 |
| Bovine serum albumin (BSA) | Fisher Scientific | Cat# BP1605100 |
| Roswell Park Memorial Institute (RPMI) 1640 with L-glutamine | Gibco | Cat# 11875-119 |
| Fetal bovine serum (FBS) | Gibco | Cat# 10437-028 |
| DPBS without Calcium and Magnesium | Fisher Scientific | Cat# 14-190-250 |
| Small Airway Epithelial Cell Growth Medium (SAGM) | PromoCell | Cat# C-21070 |
| PneumaCult-ALI Medium | STEMCELL Technologies | Cat# 05001 |
| Y-27632 2HCl | Selleck Chemicals | Cat# S1049 |
| RAPAMYCIN 99% | Fisher Scientific | Cat# R-5000 |
| CHIR 99021 | Tocris Bioscience | Cat# 4423 |
| A 83-01 | Tocris Bioscience | Cat# 2939 |
| Normocin | InvivoGen | Cat# ant-nr-1 |
| Iscove’s Modified Dulbecco’s Medium (IMDM) w/ L-glutamine and HEPES | Lonza | Cat# 12-722F |
| EDTA | Sigma-Aldrich | Cat# 03690 |
| Trypsin-EDTA (0.5%), phenol red | Thermo Scientific | Cat# 25200114 |
| Triton X-100 | Sigma-Aldrich | Cat# T8787 |
| Critical commercial assays | ||
| Red blood cell lysis buffer | Sigma-Aldrich | Cat# R7757 |
| Dithiothreitol (DTT) | Cytiva | Cat# 45-000-232 |
| Paraformaldehyde solution 4% in PBS | Santa Cruz Biotechnology | Cat# sc-281692 |
| Experimental models: Cell lines | ||
| 804G Rat Urinary System Neoplasms∗ | ATCC | CRL-11555 |
| Other | ||
| 15 mL conical tubes | Corning | Cat# 352196 |
| 50 mL conical tubes | Corning | Cat# 430291 |
| 70 μm cell strainer | CELLTREAT | Cat# 229483 |
| Hemocytometer | Bulldog Bio | Cat# DHC-N51 |
| Centrifuge | Thermo Scientific | CL2 |
| Cell culture CO2 incubator | Thermo Fisher Scientific | HEPA Class100 |
| 0.22 μm filter 500 mL vacuum filter | Corning | Cat# 430769 |
| T25 flasks | Thermo Scientific | Cat# 156367 |
| T75 flasks | Thermo Scientific | Cat# 156499 |
| Cell Culture Microscope | Nikon | Eclipse TS100 |
| Cryostar CS10 (freezing medium) | BioLife Solutions | Cat# 210102 |
| 24-well 0.4 μm insert | Corning | Cat# 3470 |
| 12-well 0.4 μm insert | Corning | Cat# 3460 |
| Thermanox Plastic Coverslip, Round | Thermo Scientific | Cat# 174950 |
| CryoPlus Storage System Liquid Nitrogen Tank | Thermo Scientific | 8207 |
| MACS column | Miltenyi Biotec | Cat# 130-042-201 |
| Microcentrifuge tubes | MTC Bio | Cat# C2000 |
| 10 mm × 10 mm × 5 mm Cryomold | VWR | Cat# 25608-922 |
| OCT medium | Sakura | Cat# 4583 |
| Fluoromount-G | SouthernBiotech | Cat# 0100-01 |
| ColorFrost Plus Microscope Slides | Thermo Fisher Scientific | Cat# 12-550-17 |
| Mr. Frosty Freezing Container | Thermo Fisher Scientific | Cat# 5100-0001 |
∗804G cells are a critical reagent. They cannot be replaced with another cell line.