REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Lamin Dm0 mouse monoclonal (1:200)	DSHB, Univ of Iowa	ADL67.10
Histone H3 rabbit polyclonal (1:500)	Abcam	ab18521
Polycomb rabbit polyclonal (1:100)	Santa Cruz Antibodies	Sc-25762
Anti-Rabbit IgG, Alexa Fluor 488 (1:500)	Thermo Fisher scientific	A-11008
Anti-Mouse IgG, Alexa Fluor 488 (1:500)	Thermo Fisher scientific	A-21202
Anti-Rabbit IgG, Alexa Fluor 594 (1:500)	Jackson ImmunoResearch	711-585-152
Anti-Mouse IgG, Alexa Fluor 594 (1:500)	Jackson ImmunoResearch	711-585-150
Chemicals, peptides, and recombinant proteins
DAPI	Sigma-Aldrich	Cat#D8417
DGD	Electron Microscopy Sciences	Cat#13255
DNase I	Sigma-Aldrich	Cat#D4527
EDTA	Sigma-Aldrich	Cat#E9884
Ethanol EM grade	Electron Microscopy Sciences	Cat#15055
Formaldehyde	Sigma-Aldrich	Cat#F8775
Glutaraldehyde EM grade	Sigma-Aldrich	Cat#G5882
HEPES	Sigma-Aldrich	Cat#H3375
Heptane	Merck	Cat#34873
Isopropanol	Sigma-Aldrich	Cat#I9516
KCl	Sigma-Aldrich	Cat#P9541
KH2PO4	Sigma-Aldrich	Cat#P5655
Na2HPO4	Sigma-Aldrich	Cat#S3264
Methanol	Merck	Cat#34860
NaCl	Sigma-Aldrich	Cat#S3041
N-butyl alcohol	Electron Microscopy Sciences	Cat#11920
Mounting media Vectashield Antifade	Vector Laboratories	Cat#H-1200-10
OsO4	Electron Microscopy Sciences	Cat#19192
PIPES	Sigma-Aldrich	Cat#P1851
PMSF	Sigma-Aldrich	Cat#P7626
Sodium Cacodylate	Electron Microscopy Sciences	Cat#11654
Sodium Hypochlorite solution	Fisher Scientific	Cat#27908
Sucrose	Sigma-Aldrich	Cat#S9378
Trizma	Sigma-Aldrich	Cat#T6066
Triton-X-100	Sigma-Aldrich	Cat#T8787
Experimental models: Organism/strains
Drosophila melanogaster, Canton-S strain	Laboratory grown	N/A
Software and algorithms
LAS X software	Leica Microsystems	Version 3.1.1.15751 (download link)
Other
Chemical fume hood	Locally fabricated	N/A
Chambered slide (2 chamber)	Nalgene Nunc	Cat#155379
Confocal microscope	Leica Microsystems	SP8 X
Coverslips	Fisherbrand	Cat#12-544B
Embryo collection cages	Locally fabricated	N/A
Fine forceps (bent)	Fine Science Tools	Cat#11274-20
Fine forceps (straight)	Fine Science Tools	Cat#11254-20
Formvar coated copper grids	Electron Microscopy Sciences	Cat#CF400-CU
Glass for knives	Electron Microscopy Sciences	Cat#7890
Glass slides	Fisherbrand	Cat#12-550-400
Heating block	Eppendorf	Thermomixer Comfort
37°C and 60°C incubator	Sanyo	MIR-262
Stereomicroscope	Carl Zeiss	Stemi 2000
Loop and Handle	Electron Microscopy Sciences	Cat#70944
Dissection Needle	Locally purchased	N/A
Paint brush	Locally purchased	N/A
pH meter	Thermo Scientific	Eutech pH700
Platform shaker	Tarsons	Rockymax Cat#4080
Rotator	Tarsons	Rotospin Cat#3070
Transmission Electron Microscope	JEOL	JEM-2100
Ultramicrotome	Leica	EM UC7
Glass cavity block	Abgil	ABG20032