Preparation of fixation buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| 10× PBS | 1× | 1 mL |
| 37% formaldehyde | 4% (v/v) | 1.08 mL |
Adjust final volume to 10 mL with deionized water. Prepare fresh before every use. Store at 25°C for the day.
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