Table 1.
Oligonucleotide primers for amplification of the complete genome of GoAstV-1 strain JXGZ by RT‐PCR.
| Primer | Sequence(5′-3′) | Nucleotide positiona | Product size(bp) |
|---|---|---|---|
| GoAstV-1 1F | CCGAAAGCGTTGGTGAGAG | 1-19 | 1552 |
| GoAstV-1 1R | GGTCCCACAGCTCATTGAAAT | 1532-1552 | |
| GoAstV-1 2F | CAGTACCACTTCAGATCTTACT | 1497-1518 | 1632 |
| GoAstV-1 2R | GCGGTTTTGGGTTCATGCAC | 3109-3128 | |
| GoAstV-1 3F | CCTTGGAGCAGAGGAAGA | 3081-3098 | 1481 |
| GoAstV-1 3R | ATGACAATCCTTCAGGTACCA | 4541-4561 | |
| GoAstV-1 4F | GAAGAGAATGTTAAGGTGCAG | 4519-4539 | 1517 |
| GoAstV-1 4R | CTGCTTAACTTGTTGGAAGATCTTT | 6011-6035 | |
| GoAstV-1 5F | GAAGATCTTCGGTGCTGCAGG | 5930-5950 | 1337 |
| GoAstV-1 5R | TTGGTTCAAAAACAGAACCG | 7247-7266 | |
| GoAstV-1 5′RACE | CCAGCATCATCACTCCAGGTT | 283-303 | |
| GoAstV-1 3′RACE | CCAGGCCTGGTCCGCTTTGA | 6948-6967 |
a
Nucleotide position is numbered based on the FLX strain (KY271027).
GoAstV-1, goose astrovirus type 1.