Table 2.
Sequences of the oligonucleotide primers used for quantitative real-time PCR.
| Gene | Accession number | Sequence (5’-3’) | Amplicon size (bp) |
|---|---|---|---|
| GAPDH | NM_204305 | Forward: AGCCATTCCTCCACCTTTGAT Reverse: AGTCCACAACACGGTTGCTGTAT |
112 |
| OCLN | NM_205128.1 | Forward: GATGGACAGCATCAACGACC Reverse: CTTGCTTTGGTAGTCTGGGC |
142 |
| CLDN-1 | NM_001013611.2 | Forward: ACACCCGTTAACACCAGATTT Reverse: GCATTTTTGGGGTAGCCTCG |
152 |
| ZO-1 | XM_040680624.1 | Forward:TCGTCGCATTGTTGAGTCTGA Reverse: TATAGCGTGTCCACAACCCG |
129 |
| MUC2 | NM_001318434 | Forward: ATTGAAGCCAGCAATGGTGT Reverse: TGACATCAGGGCACACAGAT |
214 |
Abbreviations: CLDN-1, Claudin-1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MUC2, Mucin-2; OCLN, Occludin; ZO-1, Zonula occludens-1.