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. 2022 Mar 15;101(7):101860. doi: 10.1016/j.psj.2022.101860

Figure 1.

Figure 1

Screening of primers and probe for MS detection. (A) Agarose gel electrophoresis results of RAA products with different primer pairs. M: DNA marker DL2000. 1, 3, 5: Positive reactions using primer pairs No.1-No.3, respectively; 2, 4, 6: No-template controls of primer pairs No.1-No.3, respectively. The primer pair of No.3 (MS F3/MS R3) was selected for subsequent experiments. (B) Screening of probe by RAA-LFD with the optimal primer pair MS F3/MS R3b. 1: Positive reaction using the primer-probe set MS F3/MS R3b/Probe 1; 2: No-template control of MS F3/MS R3b/Probe 1; 3: Positive reaction using the primer-probe set MS F3/MS R3b/Probe 2; 4: No-template control of MS F3/MS R3b/Probe 2. Finally, Probe 2 was selected as the optimal probe for the RAA-LFD assay. Abbreviations: MS, Mycoplasma synoviae; RAA, recombinase-aided amplification; RAA-LFD, recombinase-aided amplification-lateral flow dipstick.