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. 2022 Mar 15;101(7):101860. doi: 10.1016/j.psj.2022.101860

Figure 4.

Figure 4

Sensitivity of different methods for MS detection. (A) Sensitivity test of RAA-LFD. 1-8: RAA-LFD detection results with MS DNA template of 107–100 copies/μL, respectively; 9: Negative control. The lowest detection limit of RAA-LFD was 101 copies/μL. (B) Sensitivity test of PCR. 1-8: PCR detection results with MS DNA template of 107–100 copies/μL, respectively; 9: Negative control. The lowest detection limit of PCR was 103 copies/μL. (C) Sensitivity test of RFQ-PCR. 1–8: RFQ-PCR detection results with MS DNA template 107–10° copies/μL, respectively; 9: Negative control. The lowest detection limit of RFQ-PCR was 101 copies/μL. Abbreviations: MS, Mycoplasma synoviae; RAA-LFD, recombinase-aided amplification-lateral flow dipstick; RFQ-PCR, real-time fluorescence quantitative PCR.