Table 1.

Primers and probes used in this work.

Primers/probes	Sequences (5’-3’)	Position	Product size (bp)
MS F1	TGTTATAGCAATTTCATGTGGTGATCAAAC	60–89	171
MS R1	ACTGTACCACCTCCTGGGTTTCCTGGATTT	201–230
MS F2	ACCTGAACCAACACCTGGAAACCCAAATAC	96–125	151
MS R2	AGCCTCTACAGGGTCAACTGTACCACCTCC	217–246
MS F3	ATTACTATTAGCAGCTAGTGCAGTGGCCAT	21–50	160
MS R3	ACCTGGATTTCCTGGAGTACCTGGATTTCC	151–180
MS R3b	Biotin-ACCTGGATTTCCTGGAGTACCTGGATTTCC	151–180
Probe 1	FAM-TCATGTGGTGATCAAACTCCAGCACCTGAAC-THF-AA CACCTGGAAACCC-C3 spacer	73–119
Probe 2	FAM-TTATAGCAATTTCATGTGGTGATCAAACTCC-THF-GCA CCTGAACCAACA-C3 spacer	62–108