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. 2022 Apr 25;25(5):104300. doi: 10.1016/j.isci.2022.104300

Figure 2.

Figure 2

Aberrantly accessible chromatin in day 2 GATA6−/− populations is enriched at sites of transient GATA6 binding

(A–H); Western blot analysis for GATA6 and β-actin during early endoderm formation, quantification Figure S2A; (B) Schematic representation of doxycycline supplementation experimental plan; (C) Western blot analysis for GATA6 and β-actin at day 2 of differentiation in GATA6+/+ and GATA6−/− cells ± doxycycline; (D) Heatmaps depicting ATAC-seq signal intensity at subsets of GATA6 binding in GATA6+/+ and GATA6−/− cells ± doxycycline during early endoderm formation; (E) Heatmaps depicting GATA6 ChIP- and ATAC-seq signal intensity during early endoderm formation in GATA6+/+ and GATA6−/− cells at high confidence GATA6 occupied regions of differentially accessible chromatin identified between GATA6+/+ and GATA6−/− cells ± doxycycline at day 2 of differentiation; (F) Pie chart showing the percentage of sites with increased chromatin accessibility in GATA6+/+ or GATA6−/− cells at day 2 of differentiation that are bound by GATA6 at 1) day 2 only, 2) day 4 only, or 3) both day 2 and day 4 in wild-type cells; (G) Hypergeometric motif enrichment analysis of DNA regions enriched in chromatin that is uniquely accessible GATA6−/− cells; (H) Histograms showing density of GATA6 and EOMES motifs and their proximity to the regions of chromatin with differential accessibility between GATA6+/+ and GATA6−/− cells at day 2 of differentiation.