Figure 3.

GATA6 and EOMES co-occupancy during early endoderm formation

(A–D) Western blot analysis for GATA6 and β-actin during endoderm formation, quantification Figure S2A; (B) Western blot analysis for EOMES and β-actin during early endoderm formation, quantification Figure S2B; (C) Heatmap intensity of GATA6 and EOMES ChIP-seq signal depicting overlap of GATA6 and EOMES binding patterns at all GATA6 bound loci (left panel) and all EOMES bound loci (right panel); (D) Venn diagram of the overlap between GATA6 and EOMES peaks as determined by ChIP-seq (upper panel), heatmaps depicting GATA6 and EOMES ChIP-seq intensity within each subset (middle panel). Hypergeometric motif enrichment analysis of DNA regions uniquely enriched within each subset (lower panel).