Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Apr 25;25(5):104300. doi: 10.1016/j.isci.2022.104300

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

EOMES occupies regions of increased chromatin accessibility in GATA6^−/− cells

(A–F) RT-qPCR analysis of GATA6 and EOMES mRNA levels at day 2 of differentiation in GATA6^+/+ and GATA6^−/− cells ± doxycycline. Values normalized to the housekeeping mRNA RPL13a and shown relative to GATA6^+/+ cells, n = 3 mean ± SD; (B) Heatmap intensity of GATA6 and EOMES ChIP-seq signal at subsets of EOMES binding that are GATA6-dependent and GATA6-independent; (C) Heatmaps depicting EOMES and GATA6 ChIP- and ATAC-seq signal intensity in GATA6^+/+ and GATA6^−/− cells at GATA6 occupied regions of differentially accessible chromatin identified between GATA6^+/+ and GATA6^−/− cells ± doxycycline during early endoderm formation; (D) GO biological process analysis. Selected significantly-enriched developmental pathways related to the genes that neighbor regions of chromatin uniquely accessible in GATA6^−/− cells; (E) Genome viewer representation of the HAND1, TBXT, MESP1, and MIXL1 genomic loci aligned with GATA6 and EOMES ChIP-seq and ATAC-seq signal during early endoderm formation in GATA6^+/+ and GATA6^−/− cells; (F) mRNA expression of the genes that neighbor the selected examples of differentially accessible regions of chromatin, RNA-seq data displayed as counts per million (CPM), n = 2 mean ± SD.