Figure 2.

Short–time preventive treatment with OM–85 protects against weight loss caused by RSV infection. Mice were treated intranasally with OM–85 (1mg) 1 day prior to RSV infection. Afterwards, mice were infected with RSV (1x10⁷ PFU/ml) and received another OM–85 boost 6h later. (A) Experimental design. (B) Percentage of weight loss relative to day 0 (right before infection). (C) Total cell number and differential cell counts in BAL. (D) RSV viral load detected in lung tissue by real–time PCR (viral copies/g of lung tissue). (E) Viral titer in the lung (PFUs/g lung tissue). (F, G) Representative images of lung tissue section stained with H&E and its respective inflammation scores. Scale bars = 200 μm. Data from 2 independent experiments (n = 6 per group). All data are expressed as mean ± SEM. Multiple groups were compared using Kruskal–Wallis, except in (D, E) in which the comparison between groups was made using Mann–Whitney test. *p < 0.05.