Figure 5.
Preventive treatment with OM–85 reduces the pro–inflammatory cytokines production due RSV infection in the lungs. Mice were treated intranasally with OM–85 (1mg) 5 days prior to RSV infection (four OM–85 delivery). Afterwards, mice were infected with RSV (1x107 PFU/ml) and received another OM–85 boost 6h later. BAL and lung were harvested at day 5 post–infection. (A) Experimental design. (B) Tnfa, Il1b, Ifng, Il4, Il10, Il6 and Cxcl15 (IL–8) gene expression in the lungs detected by real–time PCR (fold change compared to untreated/uninfected control). (C) Production of TNFα, IL–1β, IFNγ, IL–4 and IL–10 in the lungs measured by ELISA. All data are expressed as mean ± SEM. Multiple groups were compared using Kruskal–Wallis. *p < 0.05.