Table 1. Overview of the Least-Squares Linear Fit Results for Normalized, Background-corrected Fluorescence and Absorbance Data.
| samplea | plate typeb | correction typec | R2 | b %d | LOD %e | z1/mm | Δzf/mm | cmaxg/μM | Amaxh (λex, λem) |
|---|---|---|---|---|---|---|---|---|---|
| Q | T (data set 1) | F1 | 0.87449 | 17.5 | 36.4 | 19.0 | 2.0 | 679.3 | 1.984, 0.158 |
| FZ | 0.99980 | 0.54 | 1.39 | ||||||
| FN | 0.99984 | 0.24 | 1.20 | ||||||
| FA | 0.95074 | –7.87 | 21.9 | ||||||
| NT (data set 2) | F1 | 0.81861 | 21.7 | 45.2 | 18.0 | 2.5 | |||
| FZ | 0.99971 | 0.12 | 1.64 | ||||||
| FN | 0.99973 | –0.08 | 1.59 | ||||||
| Q-v | T (data set 3) | F1 | 0.81967 | 21.3 | 45.1 | 19.0 | 2.0 | 316.0 | 1.873, 0.443 |
| FZ | 0.99951 | 0.95 | 2.13 | ||||||
| FN | 0.99964 | 0.43 | 1.83 | ||||||
| FA | 0.93753 | –8.15 | 24.8 | ||||||
| NT (data set 4) | F1 | 0.73752 | 25.9 | 57.3 | 18.0 | 2.0 | |||
| FZ | 0.99974 | 0.47 | 1.55 | ||||||
| FN | 0.99979 | 0.14 | 1.38 | ||||||
| Q-f | T (data set 5) | F1 | 0.98744 | 5.39 | 10.8 | 18.0 | 1.0 | 312.9 | 1.921, 0.464 |
| FZ | 0.99959 | –0.12 | 1.94 | ||||||
| FN | 0.99965 | 0.22 | 1.80 | ||||||
| FA | 0.98111 | –4.85 | 13.3 | ||||||
| NT (data set 6) | F1 | 0.98918 | 4.93 | 10.0 | 18.0 | 3.0 | |||
| FZ | 0.99964 | 1.24 | 1.83 | ||||||
| FN | 0.99972 | 0.89 | 1.61 |
Q corresponds to the pure QS concentration series; Q-v corresponds to the variable concentration of the absorber PD; Q-f corresponds to the fixed total concentration of PD.
T corresponds to the UV-transparent microplates; NT corresponds to the non-transparent microplates. Data set numbers correspond to the averaged triplicate data preformatted for automated processing.16
F1 corresponds to uncorrected fluorescence; FZ corresponds to ZINFE-corrected fluorescence intensity (eq 5); FA corresponds to absorbance IFE-corrected fluorescence intensity (eq 1); FN corresponds to NINFE-corrected fluorescence intensity.
Percent error of the normalized data slope with respect to the IFS. The values of slope and intercept used for data normalization for each concentration series are given in Table S12, Supporting Information.
LOD (α = β = 0.05); the values were normalized as percentage of cmax.
Defined as Δz = z2 – z1, where z1 and z2 are the different z-positions used for measurements of F1 and F2 (eq 5).
Maximum concentration of QS in the concentration series.
Maximum absorbance at the excitation and emission wavelengths, λex = 345 nm and λem = 390 nm, respectively.