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. 2022 Mar 12;36:03946320221079468. doi: 10.1177/03946320221079468

Figure 5.

Figure 5.

Effect of Sal B on inflammatory cytokine expression in RAW264.7 cells. (a)–(c) Gene expression of IL-1β, IL-6, and TNF-α was measured by RT-qPCR method, n = 3. (d)–(f) And production of these three cytokines was explored by ELISA kits, n = 3. (g) Immunofluorescence staining method was used to show TNF-α, magnification ×200. (h) Protein was extracted from RAW264.7 cells induced by ox-LDL. Then iNOS and VCAM were tested by Western Blotting assay. (i) The quantitative results were calculated, respectively, by ImageJ analysis software, n = 3. (j) Protein was extracted from RAW264.7 cells induced by LPS. Then iNOS, IL-6, and TNF-α were tested by Western Blotting assay. (k) The quantitative results were calculated, respectively, by ImageJ analysis software, n=3. CON, control group; ox-LDL, ox-LDL-stimulated group; LPS, LPS-stimulated group; Sal B-L, Sal B at 1.25 μg/ml concentration; Sal B-M, Sal B at 2.5 μg/ml concentration; Sal B-H, Sal B at 5 μg/ml concentration. Data are expressed as mean ± SD. *p < 0.05, **p < 0.01 vs CON group; #p < 0.05, ##p < 0.01 vs ox-LDL group or LPS group.