Average particle size |
Dynamic light scattering (DLS) and microscope technology: Scanning and transmission electron microscopy (SEM/TEM), cryogenic-TEM (Cryo-TEM), and atomic force microscopy (AFM) |
[179, 180] |
Zeta potential/Surface charge |
Electrophoretic mobility, DLS |
[181] |
Particle shape/morphology |
TEM, Cryo-TEM, and AFM |
[182] |
Lamellarity |
Cryo-TEM and 31P-NMR |
[182] |
Phase behavior |
X-ray diffraction (XRD), differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) |
[183, 184] |
Encapsulation efficiency/Drug release |
Centrifugation, dialysis followed by drug content determination using chromatographic and/or spectrophotometric methods |
[185, 186] |