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. 2022 Apr 26;56(10):6426–6435. doi: 10.1021/acs.est.1c08480

Figure 1.

Figure 1

STED-compatible dye labeling techniques (a) passive labeling with Atto 647N where the dye solution and the plastic are simply mixed together; (b) swell labeling with Atto 647N/iDye Poly Blue where the plastic is heated in the dye solution to swell the polymer matrix and allow the dye to enter the polymer matrix (with Atto 647N, THF, a solvent, is added to aid in swelling), after swelling the plastic is cooled and resuspended in DI water to deswell and remove excess dye; (c) covalent labeling by coupling NHS-functionalized Atto 647N to amine groups on functionalized plastic; (d) STED image of passively labeled 100 nm polystyrene beads with Atto 647N; (e) STED image of swell-labeled 100 nm polystyrene beads with Atto 647N; (f) STED image of swell-labeled 100 nm polystyrene beads with iDye Poly Blue; and (g) STED image of covalently labeled 100 nm PS beads with Atto 647N.