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. 2022 Apr 26;56(10):6426–6435. doi: 10.1021/acs.est.1c08480

Figure 4.

Figure 4

Longevity of different STED-compatible labeling techniques using 50 nm beads. Passive labeling with Atto 647N in the (a) pitcher plant fluid; (b) soil water; (c) elevated temperature (40 °C); and (d) hydrochloric acid (pH 2.5). Swell labeling with Atto 647N in the (e) pitcher plant fluid; (f) soil water; (g) elevated temperature (40 °C); and (h) hydrochloric acid (pH 2.5). Covalent labeling with Atto 647N in the (i) pitcher plant fluid; (j) soil water; (k) elevated temperature (40 °C); and (l) hydrochloric acid (pH 2.5). Each open circle represents the average single particle gray value for STED images of triplicate samples acquired with identical settings within treatments. Error bars represent standard errors.