FIG. 3.

Influence of sulfate on the accumulation of intracellular COS and GB. ¹³C-methyl-labeled mycelium cultured for 5 days in LPSG low-sulfate medium (shown in Fig. 2C) was transferred to fresh LPSG medium containing 5 mM Na₂SO₄ (A) or to fresh LPSG low-sulfate medium (B) as a control. The region of 72.50 to 56.80 ppm is shown with twofold enlargement in signal height in the insets. “x” placed at 58.59 and 70.22 ppm indicates the absence of signals representing the choline hydroxymethyl and N-methylene carbons, respectively.