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. 2022 May 19;22:561. doi: 10.1186/s12885-022-09596-z

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — DAB2IP negatively regulates the expression of HSP90AA1 in CRC. a The mRNA expression level of DAB2IP in human normal tissues and colorectal cancer in the TCGA database. Wilcox test was used, *P < 0.05, ** P < 0.01, ***P < 0.001 and P < 0.05 was considered statistically significant. b The mRNA expression level of HSP90AA1 in human normal tissues and colorectal cancer in the TCGA database. Wilcox test was used, *P < 0.05, ** P < 0.01, ***P < 0.001 and P < 0.05 was considered statistically significant. c The mRNA expression level of DAB2IP in human normal colon tissue and colorectal cancer in GSE8671. Wilcox test was used, *P < 0.05, ** P < 0.01, ***P < 0.001 and P < 0.05 was considered statistically significant. d The mRNA expression level of HSP90AA1 in human normal colon tissue and colorectal cancer in GSE8671. Wilcox test was used, *P < 0.05, ** P < 0.01, ***P < 0.001 and P < 0.05 was considered statistically significant. e The mRNA expression levels of DAB2IP in normal colon epithelial cells NCM460 and CRC cell lines SW480, HT29, HCT116, and DLD1 were detected by quantitative PCR. f The mRNA expression levels of HSP90AA1 in normal colon epithelial cells NCM460 and CRC cell lines SW480, HT29, HCT116, and DLD1 were detected by quantitative PCR. The above RT-PCR data were expressed as mean ± SEM, n = 3; * p < 0.05, ** p < 0.01;*** p < 0.001. g Spearman correlation analysis of the expression of DAB2IP and HSP90AA1 in the CRC dataset "Tumor Colon—Siebersmith 355-MAS5.0-U133P2" in the R2 database. h Spearman correlation analysis of DAB2IP and HSP90AA1 expression in 19 human CRC samples. i Protein expression levels of DAB2IP and HSP90AA1 proteins in 4 pairs of CRC tissues and non-tumor colon tissues of patients. j Statistical analysis of protein gray value, which was quantified by Image J software. Two-sided student’s t-tests was used, * p < 0.05, ** p < 0.01;*** p < 0.001. k l The effect of DAB2IP overexpression on the expression of HSP90AA1 was detected by PCR and Western blotting. m n Changes in HSP90AA1 expression after knocking down DAB2IP were detected by PCR and Western blotting