Table 1.

Target genes and details of the primer sequences used for quantitative SYBR^® Green real-time PCR amplification. The HPRT-1 gene was used as endogenous control gene

Target gene	GenBank accession nr	Primer sequence	Efficiency (%)	Slope	r2	Amplicon length (bp)
BD-1 [44]	NM_213838	F 5′-CAGCCACCAGCATGAGACT-3′ R 5′-CAGGTAACAGGACCATGAGGA-3′	101.47	− 3.28	0.99	63
CLDN4 [44]	AB235916	F 5’-TATCATCCTGGCCGTGCTA-3’ R 5’-CATCATCCACGCAGTTGGT-3’	100.2	− 3.29	0.99	71
OCLN [45]	FN400888	F 5’-GGAGTGATTCGGATTCTGTCTATGCT-3’ R 5’-CGCCTGGGCTGTTGGGTTGA-3’	103.8	− 3.03	0.99	423
ZO-1 [45]	CV870309	F 5′-GGCGCACGGCGAAGGTAATT-3′ R 5′-CTATCAAACTCAGGAGGCGGCACT-3’	103.7	− 3.21	0.98	405
NF-κB [44]	DQ834921	F 5’-GAAGGACCTCTAGAAGGCAAAA-3’ R 5’-GCTTTGGTTTATGCGGTGTT-3’	104.2	− 3.22	0.99	63
TNF-α [46]	NM_214022	F 5’-ACTGCACTTCGAGGTTATCGG-3’ R 5’-GGCGACGGGCTTATCTGA-3’	98.5	− 3.36	0.99	118
HPRT-1 [44]	DQ845175	F 5’-ACACTGGCAAAACAATGCAA-3’ R 5’-TGCAACCTTGACCATCTTTG-3’	102.01	− 3.25	0.99	71

HPRT-1 hypoxanthine phosphoribosyltransferase-1, CLDN4 claudin-4, OCLN occludin, ZO-1 zonula occludens-1, BD-1 β-defensin 1, NF-κB nuclear factor-κB, TNF-α tumor necrosis factor-alpha, F forward, R reverse, bp base pairs