FIG. 1.

SDS-PAGE of V. cholerae 14034 Sarkosyl-insoluble MPs that bind chitin particles in the presence or absence of GlcNAc. Electrophoresed proteins were visualized by silver staining. Molecular sizes (in kilodaltons) of markers and CBPs are indicated on the left and right, respectively. Results are shown for Sarkosyl-insoluble MPs with 100 μg of proteins loaded on the gel (A), Sarkosyl-insoluble MPs bound to chitin particles with 5 (B) or 10 (C) μg of proteins loaded on the gel, and Sarkosyl-insoluble MPs bound to chitin particles in the presence of GlcNAc (2 mg ml⁻¹) with 5 (D) or 10 (E) μg of proteins loaded on the gel, in the presence of GlcNAc (10 mg ml⁻¹) with 5 (F) or 10 (G) μg of proteins loaded on the gel, in the presence of GlcNAc (50 mg ml⁻¹) with 5 (H) or 10 (I) μg of proteins loaded on the gel, and in the presence of GlcNAc (100 mg ml⁻¹) with 5 (L) or 10 (M) μg of proteins loaded on the gel.