TABLE 1.
Effects of treatment of bacteria with pronase E, sodium m-periodate, and sugars on V. cholerae attachment to chitin
| Strain | Mean no. of bacteria (106) per 2.5 mg of chitin ± SD (% inhibition)a treated with:
|
||||||
|---|---|---|---|---|---|---|---|
| Nothing (control) | Pronase E | Na m-periodate | GlcNAc | Chitobiose | Chitotriose | d-Glucoseb | |
| 14034 | 37 ± 0.8 | 11 ± 0.5 (70) | 37 ± 1.4 (NI) | 14 ± 0.8 (62) | 32 ± 1.2 (14) | 29 ± 0.5 (22) | 37 ± 0.5 (NI) |
| 14035 | 39 ± 2.1 | 12 ± 0.8 (69) | 39 ± 1.6 (NI) | 15 ± 0.8 (62) | 33 ± 1.6 (15) | 30 ± 1.7 (23) | 39 ± 1.7 (NI) |
a
Results are averages of three independent experiments performed with three cultures on different days. Percentages of attachment inhibition were calculated by comparing the treated samples with untreated controls. NI, no inhibition. Differences between samples treated with GlcNAc, chitobiose, chitotriose, and pronase E and untreated controls were statistically significant (P ≤ 0.05).
b
Results similar to those obtained with d-glucose were obtained with d-fucose and d-fructose.