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. 2022 May 19;12:8469. doi: 10.1038/s41598-022-12242-0

Table 1.

Panning strategy for isolating specific antibodies against the SARS-CoV-2 N protein.

Round Coating antigen concentration (μg) Input (cfu) Blocking buffer Washing times of PBST
1st 10 1 × 1012 4% skim milk 15
2nd 2 5 × 1011 4% skim milk 25