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. 2022 Apr 9;298(5):101917. doi: 10.1016/j.jbc.2022.101917

Figure 4.

Figure 4

AM0627 point mutations alter cleavage patterns.A-F, fetuin (A), recombinant MUC16 (B), recombinant PSGL-1 (C), purified C1-INH (D), recombinant podocalyxin (E), and recombinant CD43 (F) were incubated with 50 nM AM0627 or AM0627 point mutants ± 1 U SialEXO for 22 h at 37 °C. Digests were separated by SDS-PAGE and visualized using Coomassie stain. Gels were spliced to remove an inactive AM0627 variant that was tested. C1-INH, C1 esterase inhibitor; MUC16, mucin 16; PSGL-1, P-selectin glycoprotein ligand 1.