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. 2022 Apr 12;298(5):101932. doi: 10.1016/j.jbc.2022.101932

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© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Characterization of GPR84 phospho-site-specific antisera. Flp-In TREx 293 cells harboring human GPR84-eYFP (-dox) or induced to express the receptor construct (+dox) were treated with Lambda protein phosphatase (λ-PPase), 2-HTP, and/or compound 101 (cmp101) as indicated. Following enrichment of the receptor construct via GFP-trapping and SDS-PAGE immunoblots were performed with the C-terminal GPR84 structural antiserum (A and D) or the phospho-site-targeted antisera pSer²²¹/pSer²²⁴ (B and E), and pThr²⁶³/pThr²⁶⁴ (C and F). Apparent molecular mass markers are shown, and mature and immature forms of GPR84 are highlighted. Illustrative outcomes are displayed. dox, doxycycline.