Figure 9.

DL-175 does not require Arg¹⁷²for function at GPR84. The ability of various concentrations of 2-HTP (A), 6-OAU (B), PSB-16671 (C), and DL-175 (D) to stimulate binding of [³⁵S]GTPγS was assessed in membranes of Flp-In TREx 293 induced to express wildtype, Arg¹⁷²Ala, or Arg¹⁷²Lys human GPR84-Gα_i2 fusion proteins. The ability of various concentrations of 2-HTP (E), 6-OAU (F), and DL-175 (G) to stimulate arrestin-3 recruitment was assessed in cells transfected with either wildtype or Arg¹⁷²Ala GPR84-eYFP. Data are means ± SEM. n ≥ 3.