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Observed m/z 29 Versus m/z 30 Ion Ratios for Both Urea and Biuret at Three pH Values Tested and the Corresponding Percentage of Intramolecular Reaction Leading to N2 Formation
| Condition | Ratio of m/z 29/30 | Percentage of intramolecular N2 formation |
|---|---|---|
| 1 mM urea–pH 5 | 1.78 | 5.8 |
| 1 mM urea–pH 7 | 1.76 | 6.3 |
| 1 mM urea–pH 10 | 0.93 | 36.6 |
| 1 mM biuret–pH 5 | 1.99 | 0.3 |
| 1 mM biuret–pH 7 | 1.97 | 1.1 |
| 1 mM biuret–pH 10 | 1.44 | 24.6 |
