FIG 1.

Summary of current WHO-designated VOC along with their expected spike mutations at sites targeted by this two-reaction multiplex SARS-CoV-2 RT-qPCR genotyping approach. These reactions are designed to detect the following mutations: del69–70, K417N, L452R, T478K, E484K, and N501Y. Shading indicates predicted versus empirical performance of this assay for the detection and differentiation of these VOCs. The predicted detection for the VOC Omicron is based on both the sequence at the target sites and known adjacent mutations in the probe binding site. *, Known limitation of the assay in differentiating VOC Beta from the VOI Mu; **, known limitation of the assay in differentiating VOC Gamma from the VOI Mu.