TABLE 1.
Reaction 2 primer and probe oligonucleotide sequences
| Namea | Sequence (5′→3′) | Final concn (nM) |
|---|---|---|
| Primers | ||
| del69–70_FWD | CATTAAATGGTAGGACAGGGTTA | 300 |
| del69–70_REV | ACATTCAACTCAGGACTTGTT | 300 |
| K417N_FWD | GCAGCCTGTAAAATCATCTG | 300 |
| K417N_REV | CATTTGTAATTAGAGGTGATGAAGTC | 300 |
| T478K_FWD | AAAGGAAAGTAACAATTAAAACCT | 300 |
| T478K_REV | AGGAAGTCTAATCTCAAACCT | 300 |
| Probes | ||
| del69–70_MT_HEX | HEX-TTGGTCCCAGAGATAGCATG-BHQ1 | 50 |
| wt69–70_WT_CY3.5b | CY3.5-GGTCCCAGAGACATGTATAG-BHQ2 | 50 |
| K417N_MT_CY5 | CY5-TAATCAGCAATATTTCCAGT-BHQ2 | 50 |
| T478K_MT_FAM | FAM-ACCATTACAAGGTTTGCTAC-BHQ1 | 50 |
a
FWD, forward; REV, reverse; WT, wild-type; MT, mutant; CY3.5, cyanine 3.5; BHQ, black hole quencher.
b
Included as an internal amplification control for samples without the del69–70 mutation.