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. 2022 May 5;9:897264. doi: 10.3389/fmed.2022.897264

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Copyright © 2022 Gutowska, McKinnon, Sarkis, Doster, Bissa, Moles, Stamos, Rahman, Washington-Parks, Davis, Yarchoan, Franchini and Pise-Masison.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell-mediated responses in Pom-treated animals. (A) Representative flow cytometric pseudocolor plots showing the frequency of IFN-γ producing CD4⁺ (upper) and CD8⁺ (lower) T cells in bone marrow from unstimulated cultures or after stimulation with HTLV-1 Tax/Gag peptide pools or after stimulated with PMA/ionomycin. (B) Plots of the frequency (%) of cytokine producing CD4⁺ cells over the course of the study are shown for each animal. Colors for individual cytokines/granzyme B (GranB) are indicated in the figure. The upper panels represent cultures stimulated with a peptide pool of overlapping 15-mer peptides spanning the HTLV-1 Tax and Gag proteins. The lower panels represent cultures stimulated with PMA/ionomycin.