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Final SOP parameters for the optimal detection of hCoV infection by flow cytometry.
| Virus | Cell line | Days post infection | MOI | Primary antibody | Secondary antibody |
|---|---|---|---|---|---|
| HCoV-229E | Huh-7 | 2 | 0.028 | 1:5,000 | 1:5,000 |
| HCoV-NL63 | Caco-2 | 3 | 0.018 | 1:5,000 | 1:5,000 |
| HCoV-OC43 | Huh-7 | 3 | 0.008 | 1:5,000 | 1:5,000 |
