Table 2.

Final SOP parameters for the optimal detection of hCoV infection by in-cell ELISA.

Virus	Cell line	Days post infection	MOI	Primary antibody	Secondary antibody	Z′ factor	S/N ratio
HCoV-229E	Huh-7	2	0.002	1:5,000	1:15,000	0.88	22.56
HCoV-NL63	Caco-2	6	0.01	1:5,000	1:10,000	0.83	6.81
HCoV-OC43	Huh-7	3	0.006	1:5,000	1:10,000	0.92	21.75