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. 2021 Jul 15;6(61):eabh3634. doi: 10.1126/sciimmunol.abh3634

Fig. 8. The RBD + 3M-052-alum vaccine in comparison with RBD + alum induces increased frequencies of blood ASCs and up-regulation of tissue homing markers.

Fig. 8.

(A) Flow plots highlight CD38+CD80+ ASCs (gating strategy in fig. S12) from one animal each vaccinated with RBD + alum or RBD + 3M-052-alum adjuvants at week 9 and week 9 + day 4. FITC, fluorescein isothiocyanate. (B) Scatterplots summarize frequencies of blood ASCs before and after vaccination at week 9. Scatterplots in (C) to (F) summarize the change in differential geometric mean fluorescence intensity (MFI) (stain isotype) of chemokine receptors CXCR3, CXCR4, CCR7, and alpha4:beta7 on blood ASCs, before and day 4 after vaccination at week 9 in the study. (G) Correlation of flow-based frequencies of total blood ASCs with RBD-specific ELISpot–based IgG+ ASCs at week 9 + day 4 in the study is shown. (H) Correlation of RBD-specific IgG+ blood ASCs (week 9 + day 4) with IgG+ ASCs in draining LNs at termination after challenge is shown. (I) Correlation of RBD-specific IgG+ blood ASCs (week 9 + day 4) with IgG+ ASCs in BM aspirates at week 11 is shown. (J) Correlation of RBD-specific IgG+ blood ASCs (week 9 + day 4) with IgG+ ASCs in femur BM scoop tissue at necropsy is shown. Statistical significance of the difference in responses measured was tested using a nonparametric two-tailed Mann-Whitney test. P < 0.05 was used as significant. **P = 0.0079 in (B), *P = 0.03 in (C), and *P = 0.0159 in (D).