CRISPR Repair Reveals Causative Mutation in a Preclinical Model of Retinitis Pigmentosa: A Brief Methodology

. Author manuscript; available in PMC: 2022 May 19.

Published in final edited form as: Methods Mol Biol. 2018;1715:191–205. doi: 10.1007/978-1-4939-7522-8_13

Ingredients	Quantity (per reaction, μL)	Final
Annealed heteroduplex	X	200–400 ng
SURVEYOR nuclease S	1
SURVEYOR enhancer S	1
MgCl₂ solution supplied with kit, 0.15 M	0.1×
Total	Y (10–40)