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Oligo sequences for Pde6b sgRNA construction
| sgRNA oligos | Sequence (5′ → 3′) |
|---|---|
| sgRNA1-foward | CACCGCCACTTTCTGCTACTTAGGT |
| sgRNA1-reverse | AAACACCTAAGTAGCAGAAAGTGGC |
| sgRNA2-foward | CACCGCTCCAGGCCTTGGCCTGTAC |
| sgRNA2-reverse | AAACGTACAGGCCAAGGCCTGGAGC |
| sgRNA3-foward | CACCGAGGGCCCAGTGATCAGCCGG |
| sgRNA3-reverse | AAACCCGGCTGATCACTGGGCCCTC |
| sgRNA4-foward | CACCGCCAACCTAAGTAGCAGAAAG |
| sgRNA4 reverse | AAACCTTTCTGCTACTTAGGTTGGC |
